CDC27抗体

WB analysis of HeLa whole cell lysate using GTX41386 CDC27 antibody.
Loading : 35 μg
Dilution : 1 _x0010_μg/mL

Western blot using Affinity Purified anti-cdc27 antibody (GTX41386) shows detection of a band ~90 kDa corresponding to human cdc27 (arrowhead). Approximately 35 ug of HeLa whole cell lysate was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 ug/ml for 2 h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye800 fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.