Coxsackievirus A6 VP1抗体 [HL3698]

Coxsackievirus A6 VP1 antibody [HL3698] detects Coxsackievirus A6 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Coxsackievirus A6 VP1 stained by Coxsackievirus A6 VP1 antibody [HL3698] (GTX641870) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Coxsackievirus A6 VP1 antibody [HL3698] (GTX641870) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) RD whole cell extracts (5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Coxsackievirus A6 VP1 antibody [HL3698] (GTX641870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.