PYK2抗体

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

PYK2 antibody detects PYK2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PYK2 stained by PYK2 antibody (GTX130887) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

PYK2 antibody detects PYK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PYK2 protein stained by PYK2 antibody (GTX130887) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PYK2 antibody (GTX130887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PYK2 antibody detects PYK2 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
PYK2 antibody (GTX130887) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of PYK2 protein from A431 whole cell extracts using 5 μg of PYK2 antibody (GTX130887).
Western blot analysis was performed using PYK2 antibody (GTX130887) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.